Human PSCA-Protein (PCA-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PSCA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PSCA Antibody, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu12-Ser86

Catalog: PCA-HM201

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Product Description	Recombinant Human PSCA-Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu12-Ser86.
Product Background	Gastric cancer is a deadly malignancy and is a prognostically unfavorable entity with restricted therapeutic strategies available. Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein widely expressed in bladder, prostate, and pancreatic cancers. Existing studies have thoroughly recognized the availability of utilizing anti-PSCA CAR-T cells in the treatment of metastatic prostate cancer and non-small-cell lung cancer.
Product Category	Recombinant Protein

Target	PSCA
Synonyms	PSCA; UNQ206; PRO232
Gene/Protein ID	O43653
Species	Human
Exact Sequence	Leu12-Ser86
Molecular Weight	The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PSCA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PSCA Antibody, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PSCA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PSCA Antibody, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.
	The purity of Human PSCA-is greater than 95% as determined by SEC-HPLC.
	Human PSCA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.