Human R spondin 3/RSPO3 Protein (RSP-HM103)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human R-Spondin 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln22-Val146

Catalog: RSP-HM103

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Product Description	Recombinant Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val146.
Product Background	The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Product Category	Recombinant Protein

Target	R spondin 3/RSPO3
Gene/Protein ID	Q9BXY4-1
Species	Human
Exact Sequence	Gln22-Val146
Molecular Weight	The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human R-Spondin 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).
	Immobilized Human R-Spondin 3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA (QC Test).
	Human R spondin 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.