Human RANKL/TNFSF11/CD254 Protein (RKL-HM101)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 90% as determined by Tris-Bis PAGE
Activity: Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly63-Asp244

Catalog: RKL-HM101

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Product Description	Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244.
Product Background	Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
Product Category	Recombinant Protein

Target	RANKL/TNFSF11/CD254
Synonyms	ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Gene/Protein ID	O14788-2
Species	Human
Exact Sequence	Gly63-Asp244
Molecular Weight	The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 90% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
	Human Rankl on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.