Human SCF Protein (SCF-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu26-Ala189

Catalog: SCF-HE001

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Product Description	Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.
Product Background	Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Product Category	Recombinant Protein

Target	SCF
Gene/Protein ID	P21583-1
Species	Human
Exact Sequence	Glu26-Ala189
Molecular Weight	The protein has a predicted MW of 18.45 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6ug/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6ug/ml determined by ELISA.
	Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test).
	Human SCF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.