Human SEMA3A Protein (SEM-HM13A)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asn21-Val771

Catalog: SEM-HM13A

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Product Description	Recombinant Human SEMA3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn21-Val771.
Product Background	The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.
Product Category	Recombinant Protein

Target	SEMA3A
Synonyms	Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; semaphorin L
Gene/Protein ID	Q14563
Species	Human
Exact Sequence	Asn21-Val771
Molecular Weight	The protein has a predicted MW of 87.7 kDa. Due to cleavage site, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-SEMA3A Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30ug/ml determined by ELISA.
	Human SEMA3A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.