Human SERPINF2/A2AP Protein (SPF-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Met28-Lys491

Catalog: SPF-HM101

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Product Description	Recombinant Human SERPINF2/A2AP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met28-Lys491.
Product Background	Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP; SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.
Product Category	Recombinant Protein

Target	SERPINF2/A2AP
Synonyms	Alpha-2-AP; AAP; PLI; SERPINF2; A2AP; ALPHA-2-PI; API; Serpin F2
Gene/Protein ID	P08697-1
Species	Human
Exact Sequence	Met28-Lys491
Molecular Weight	The protein has a predicted MW of 52.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human SERPINF2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA.
	The purity of Human SERPINF2 is greater than 90% as determined by SEC-HPLC.
	Human SERPINF2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.