Human SFRP2 Protein (SRP-HM102)
Catalog: SRP-HM102
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Product Description | Recombinant Human SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295. |
Product Background |
As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients. |
Product Category |
Recombinant Protein |
Target |
SFRP2 |
Synonyms | sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2; AI851596 |
Gene/Protein ID |
Q96HF1 |
Species |
Human |
Exact Sequence |
Leu25-Cys295 |
Molecular Weight | The protein has a predicted MW of 32.4 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 20mM PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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