Human SG3/Secretogranin 3 Protein (SGS-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SG3, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe20-Leu468

Catalog: SGS-HM101

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Product Description	Recombinant Human SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Leu468.
Product Background	Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.
Product Category	Recombinant Protein

Target	SG3/Secretogranin 3
Synonyms	Secretogranin-3; Secretogranin III; SCG3; SGIII; UNQ2502/PRO5990
Gene/Protein ID	Q8WXD2-1
Species	Human
Exact Sequence	Phe20-Leu468
Molecular Weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SG3, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human SG3, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA.
	The purity of Human SG3 is greater than 95% as determined by SEC-HPLC.
	Human SG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.