Human TNFRSF11B Protein (TNF-HM11B)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu22-Leu401

Catalog: TNF-HM11B

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Product Description	Recombinant Human TNFRSF11B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Leu401.
Product Background	Osteoprotegerin (OPG) is a key regulator in bone metabolism, that also has effect in vascular system. Studies suggest that osteoprotegerin is a critical arterial calcification inhibitor, and is released by endothelial cells as a protective mechanism for their survival in certain pathological conditions, such as diabetes mellitus, chronic kidney disease, and other metabolic disorders.
Product Category	Recombinant Protein

Target	TNFRSF11B
Gene/Protein ID	O00300
Species	Human
Exact Sequence	Glu22-Leu401
Molecular Weight	The protein has a predicted MW of 44.71 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
	Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.8ng/ml determined by ELISA (QC Test).
	Human TNFRSF11B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.