Human TNFRSF12A/TWEAKR Protein (TNF-HM20A)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human TNFRSF12A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu28-Per80

Catalog: TNF-HM20A

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Glu28-Per80.
Product Background	Thyroid cancer (TC) is the most well-known endocrine neoplasia as well as a common malignant tumor in the head and neck.TNFRSF12A expression may be a potential useful prognostic molecular biomarker of bad survival in thyroid cancer, in addition, PPAR signaling pathway, insulin signaling pathway, mTOR signaling pathway may be the key pathway controlled by TNFRSF12A in thyroid cancer. Further experimental ought to be performed to demonstrate the biologic effect of TNFRSF12A.
Product Category	Recombinant Protein

Target	TNFRSF12A/TWEAKR
Synonyms	FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
Gene/Protein ID	Q9NP84-1
Species	Human
Exact Sequence	Glu28-Per80
Molecular Weight	The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TNFRSF12A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFRSF12A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
	Human TNFRSF12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.