Human TNFSF15 Protein (FSF-HM115)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 12.00 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu72-Leu251

Catalog: FSF-HM115

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu72-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein

Target	TNFSF15
Gene/Protein ID	O95150-1
Species	Human
Exact Sequence	Leu72-Leu251
Molecular Weight	The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 12.00 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15, His Tag with an affinity constant of 12.00 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.