Human TSLPR Protein (TSP-HM20R)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TSLP (R127A, R130A) , His Tag at 1ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Gly25-Lys231

Catalog: TSP-HM20R

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Product Description	Recombinant Human TSLPR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly25-Lys231.
Product Background	Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
Product Category	Recombinant Protein

Target	TSLPR
Synonyms	TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
Gene/Protein ID	Q9HC73-1
Species	Human
Exact Sequence	Gly25-Lys231
Molecular Weight	The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TSLP (R127A, R130A) , His Tag at 1ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TSLP (R127A, R130A) , His Tag at 1ug/ml (100ul/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA (QC Test).
	Immobilized Human TSLPR, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.
	The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
	Human TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.