• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 8.70 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly49-Ala118
Catalog #IGF-HE001
Price
Regular price $24.00
Regular price Sale price $24.00
/
Size
Avantor VWR Fisher Scientific

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Human IGFI Protein (IGF-HE001) Summary

Product Description

 Recombinant Human IGFI Protein is expressed from E.coli without tag.It contains Gly49-Ala118.

Product Background

 The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IGFI Protein (IGF-HE001) Specifications/Details

Protein
 IGFI
Synonyms
 IGF-I; MGF; IBP1; IGF1A;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly49-Ala118
Molecular Weight
 The protein has a predicted MW of 7.65 kDa. The protein migrates to 8-12 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 8.70 nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag

Fluorophore

 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IGF1R, His Tag captured on CM5 Chip via Protein A can bind Human IGFI, No Tag with an affinity constant of 8.70 nM as determined in SPR assay (Biacore T200). Human IGF1R, His Tag captured on CM5 Chip via Protein A can bind Human IGFI, No Tag with an affinity constant of 8.70 nM as determined in SPR assay (Biacore T200).
Human IGFI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IGFI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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