• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-12, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
  • Amino Acid Range: Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Catalog #IL1-HM412B
Price
Regular price $82500
Regular price Sale price $82500
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-12 Protein (IL1-HM412B) Summary

Product Description

 Recombinant Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) and Arg23-Ser219 (IL-12A).

Product Background

 Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Biotinylated Human IL-12 Protein (IL1-HM412B) Specifications/Details

Protein
 IL-12
Synonyms
 CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12;IL12B; IL12p70; IL12 subunit p35;Interleukin-12; IL-12; IL 12
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Molecular Weight
 The protein has a predicted MW of 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-12, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human IL-12, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. Immobilized Biotinylated Human IL-12, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Biotinylated Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-12 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human IL-12 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...