Name: Human IL-3 Protein (IL3-HM201)
Brand: KactusBio
SKU: IL3-HM201-100UG
Price: 247.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala20-Phe152

Catalog #IL3-HM201

Size

100UG

500UG

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Human IL-3 Protein (IL3-HM201) Summary

Product Description	Recombinant Human IL-3 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala20-Phe152.
Product Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-3 Protein (IL3-HM201) Specifications/Details

Protein	IL-3
Synonyms	IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession	P08700
Species	Human
Biotinylated	no
Amino Acid Range	Ala20-Phe152
Molecular Weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).
	Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-3, hFc Tag Tag on ProA-Biosensor, can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.55 µM as determined in BLI assay (Gator).
	The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.

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