• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu28–Leu396
Catalog #LGR-HM104
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human LGR-4 Protein (LGR-HM104) Summary

Product Description

 Recombinant Human LGR-4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu28–Leu396.

Product Background

 Leucine-rich repeat-containing G protein-coupled receptor (LGR)-4 is a G protein-coupled receptor (GPCR) with a seven-transmembrane domain structure. LGRs are evolutionally and structurally phylogenetic, classified into three subgroups and are members of the so-called orphan receptors.

Human LGR-4 Protein (LGR-HM104) Specifications/Details

Protein
 LGR-4
Synonyms
 LGR4; GPR48
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu28–Leu396
Molecular Weight
 The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. The affinity constant of 0.440 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR4, His Tag with an affinity constant of 0.440 uM as determined in SPR assay (Biacore T200). Human R Spondin 3, His Tag immobilized on CM5 Chip can bind Human LGR4, His Tag with an affinity constant of 0.440 uM as determined in SPR assay (Biacore T200).
Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. Immobilized Human LGR-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LGR-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Human LGR-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LGR-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LGR-4 is greater than 95% as determined by SEC-HPLC. The purity of Human LGR-4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...