Human MFAP4 Protein (MAP-HM104) Summary
Product Description |
Recombinant Human MFAP4 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val22-Ala255. |
Product Background |
Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human MFAP4 Protein (MAP-HM104) Specifications/Details
Protein |
MFAP4 |
Synonyms |
Microfibril-associated glycoprotein 4;Mfap4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val22-Ala255 |
Molecular Weight |
The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MFAP4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human MFAP4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. |
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Human MFAP4 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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The purity of Human MFAP4 is greater than 95% as determined by SEC-HPLC. |
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