Name: Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein (MHC-HM412B)
Brand: KactusBio
SKU: MHC-HM412B-100UG
Price: 341.0 USD
Availability: InStock

HPLC chromatogram showing purity analysis of recombinant protein for quality control validation.

Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein (MHC-HM412B)

Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and LMLGEFLKL peptide

Catalog #MHC-HM412B

Size

100UG

500UG

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Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein (MHC-HM412B) Summary

Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&Survivin (LMLGEFLKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01),Ile21-Met119(B2M) and LMLGEFLKL peptide.
Product Background	Survivin (also known as BIRC5) is an evolutionarily conserved eukaryotic protein that is essential for cell division and can inhibit cell death. Normally it is only expressed in actively proliferating cells, but is upregulated in most, if not all cancers; consequently, it has received significant attention as a potential oncotherapeutic target.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Specifications

Protein	HLA-A*02:01&B2M&Survivin (LMLGEFLKL)
Synonyms	Survivin; MHC; MHC I; BIRC5; API4; EPR-1; IAP4; survivin variant 3 alpha
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&LMLGEFLKL
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and LMLGEFLKL peptide
Molecular Weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&Survivin (LMLGEFLKL) Monomer is greater than 95% as determined by SEC-HPLC.