Name: Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T)
Brand: KactusBio
SKU: MHC-HM443T-100UG
Price: 825.0 USD
Availability: InStock

HPLC chromatogram showing a sharp protein purity peak at 8.741 minutes with baseline separation from 6.184 to 8.779 minutes.

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T)

Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA.

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.

Loaded HLA-A*02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay (Gator® Prime).

The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide

Catalog #MHC-HM443T

Size

100UG

500UG

Order now, ships by:

[{"id":43911546372318,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"MHC-HM443T-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human HLA-A*02:01\u0026B2M\u0026PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T) - 100UG","public_title":"100UG","options":["100UG"],"price":82500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43911552794846,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"MHC-HM443T-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Human HLA-A*02:01\u0026B2M\u0026PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T) - 500UG","public_title":"500UG","options":["500UG"],"price":330000,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T) Summary

Product Description	Recombinant Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide.
Product Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Specifications

Protein	HLA-A*02:01&B2M&PRAME (SLLQHLIGL)
Synonyms	PRAME; OIP-4; OIP4; MAPE;
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913 (HLA-A*02:01)&P61769 (B2M)&SLLQHLIGL
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA.
	Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
	Loaded HLA-A02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay (Gator® Prime).
	The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC.