• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide
Catalog #MHC-MM452
Price
Regular price $34100
Regular price Sale price $34100
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Size
Avantor VWR Fisher Scientific

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Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein (MHC-MM452) Summary

Product Description

 Recombinant Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide.

Product Background

 Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells.

Q1-1b&B2M&Qdm (AMAPRTLLL) Specifications

Protein
 Q1-1b&B2M&Qdm (AMAPRTLLL)
Synonyms
 Qdm; Qa-1b; MHC; H2-T23;Qa1b; H2T23
MHC Form
 Monomer
Allele
 Qa-1b
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide
Molecular Weight
 The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
Other Available Proteins:
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