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Non-biotinylated Human MMP-9 Protein (pro form) (MMP-HM409) Summary
Product Description |
Recombinant Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA for an activated form.It contains Ala20-Asp707. |
Product Background |
Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Non-biotinylated Human MMP-9 Protein (pro form) (MMP-HM409) Specifications/Details
Protein |
MMP-9 |
Synonyms |
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala20-Asp707 |
Molecular Weight |
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MMP-9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 1500 pmol/min/µg. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35 (w/v) (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human MMP-9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. |
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Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human MMP-9 is greater than 90% as determined by SEC-HPLC. |
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