Mouse APRIL/TNFSF13 Protein (APR-MM113)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala96-Leu241

Catalog: APR-MM113

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Product Description	Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala96-Leu241.
Product Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Product Category	Recombinant Protein

Target	APRIL/TNFSF13
Gene/Protein ID	Q9D777
Species	Mouse
Exact Sequence	Ala96-Leu241
Molecular Weight	The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
	The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC.
	Mouse APRIL on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.