Mouse CD117 Protein (CD1-MM117)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 14.02 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser25-Pro523

Catalog: CD1-MM117

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Product Description	Recombinant Mouse CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser25-Pro523.
Product Background	The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Product Category	Recombinant Protein

Target	CD117
Gene/Protein ID	P05532-2
Species	Mouse
Exact Sequence	Ser25-Pro523
Molecular Weight	The protein has a predicted MW of 56.63 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 14.02 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 uM as determined in SPR assay (Biacore T200).
	The purity of Mouse CD117 is greater than 95% as determined by SEC-HPLC.
	Mouse CD117 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.