Mouse Fc gamma RIV/CD16-2 Protein (FCR-MM162)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly21-Gln203

Catalog: FCR-MM162

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Product Description	Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly21-Gln203.
Product Background	FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation.
Product Category	Recombinant Protein

Target	Fc gamma RIV/CD16-2
Synonyms	CD16-2; FcgammaRIV; Fcgr4
Gene/Protein ID	A0A0B4J1G0-1
Species	Mouse
Exact Sequence	Gly21-Gln203
Molecular Weight	The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 146 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
	The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC.
	Mouse Fc gamma RIV on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.