Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453)
Catalog: MHC-MM453
Order now get it on
| Product Description | Recombinant Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide. |
Product Background |
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature. |
Product Category |
Recombinant Protein |
Target |
H-2K(b)&B2M&OVA (SIINFEKL) Monomer |
| MHC Form | Monomer |
| Allele | H-2K (b) |
Gene/Protein ID |
P01901(H-2K(b))&P01887(B2M)&SIINFEKL |
Species |
Mouse |
Exact Sequence |
His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide |
| Molecular Weight | The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
The purity of Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer is greater than 95% as determined by SEC-HPLC. |
|
|
Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products