Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Val20-Pro211

Catalog: OX4-MM440

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Product Description	Recombinant Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211.
Product Background	Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Product Category	Recombinant Protein

Target	OX40/TNFRSF4/CD134
Synonyms	CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16
Gene/Protein ID	P47741
Species	Mouse
Exact Sequence	Val20-Pro211
Molecular Weight	The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA.
	The purity of Mouse OX40 is greater than 90% as determined by SEC-HPLC.
	Mouse OX40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.