Mouse SPP1/OPN Protein (SPP-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Leu17-Asn294

Catalog: SPP-MM101

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Product Description Recombinant Mouse SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu17-Asn294.

Product Background

 Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.

Product Category

 Recombinant Protein

Target

 SPP1/OPN
Synonyms Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI

Gene/Protein ID

 P10923

Species

 Mouse

Exact Sequence

 Leu17-Asn294
Molecular Weight The protein has a predicted MW of 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Mouse SPP1 is greater than 95% as determined by SEC-HPLC.
Mouse SPP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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