Mouse VCAM-1/CD106 Protein (VAM-MM106)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Phe25-Glu698

Catalog: VAM-MM106

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Product Description Recombinant Mouse VCAM-1/CD106 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu698.

Product Background

 Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.

Product Category

 Recombinant Protein

Target

 VCAM-1/CD106

Gene/Protein ID

 NP_035823.3

Species

 Mouse

Exact Sequence

 Phe25-Glu698
Molecular Weight The protein has a predicted MW of 75.48 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to Mouse VCAM-1 coated plates (10 ug/ml with 100 ul/well) , approximately 70 % - 80 % cells will adhere after 1 hour incubation at room temperature.
The purity of Mouse VCAM-1 is greater than 95% as determined by SEC-HPLC.
Mouse VCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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