mRNA Cap 2'-O-Methyltransferase, GMP-Grade (GMP-MEH-VE101), DMF #038029
Catalog: GMP-MEH-VE101
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| Product Description | mRNA Cap 2'-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure. Cap1 structure is a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5' triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5. NOTE: Vaccinia Capping Enzyme #GMP-VCS-VE101) is required to generate Cap0 structure. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038029. |
| Application | RNA Modification |
| Concentration | 50U/µL |
| Unit Definition | One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C. |
| Source | Expressed in an E.coli strain with the gene of mRNA Cap 2'-O-Methyltransferase. |
| Quality Standards | Activity (Capping modification and efficiency measurement): ≥ 67.7 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual DNase: Negative Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 5 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host Protein: ≤ 20 ng/mg Residual Heavy Metal: ≤ 10 ppm Residual Nickel Salt: ≤ 10 ppm Bioburden: ≤ 1 CFU/10mL |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. Analysis of mRNA capping efficiency using LC-MS. |
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