Regular price
$346.50
Order by 4pm ET for delivery on:
Non-biotinylated Human Nectin-3/CD113 Protein (NEC-HM403) Summary
Product Description |
Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400. |
Product Background |
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human Nectin-3/CD113 Protein (NEC-HM403) Specifications/Details
Protein |
Nectin-3/CD113 |
Synonyms |
CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly58-Asp400 |
Molecular Weight |
The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.204 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). |
|
|
Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
