Rat Coagulation Factor II-Protein (CFR-RM102)
Catalog: CFR-RM102
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| Product Description | Recombinant Rat Coagulation Factor II-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617. |
Product Background |
Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites. |
Product Category |
Recombinant Protein |
Target |
Coagulation Factor II |
Gene/Protein ID |
NP_075213.2 |
Species |
Rat |
Exact Sequence |
Gln25-Arg617 |
| Molecular Weight | The protein has a predicted MW of 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Rat Coagulation Factor II is greater than 95% as determined by SEC-HPLC. |
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Rat Coagulation Factor II on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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