• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Catalog #RSV-VM1PR
Price
Regular price $74250
Regular price Sale price $74250
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Size
Avantor VWR Fisher Scientific

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HRSV (A, strain A2) Pre-fusion glycoprotein F0 Protein (RSV-VM1PR) Summary

Product Description

 Recombinant HRSV (A, strain A2) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.

Product Background

 Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.

HRSV (A, strain A2) Pre-fusion glycoprotein F0 Protein (RSV-VM1PR) Specifications/Details

Protein
 Pre-fusion glycoprotein F0
Synonyms
 Pre-fusion glycoprotein;
Species
 HRSV (A, strain A2)
Biotinylated
 no
Molecular Weight
 The protein has a predicted MW of 56.28 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (5C4) Antibody, hFc Tag with the EC50 of 64.4ng/ml determined by ELISA. Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (5C4) Antibody, hFc Tag with the EC50 of 64.4ng/ml determined by ELISA.
Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
HRSV (A, strain A2) Pre-fusion glycoprotein F0 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. HRSV (A, strain A2) Pre-fusion glycoprotein F0 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of HRSV (A, strain A2) Pre-fusion glycoprotein F0 is greater than 95% as determined by SEC-HPLC. The purity of HRSV (A, strain A2) Pre-fusion glycoprotein F0 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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