SEC-HPLC chromatogram of recombinant human Siglec-4a/MAG protein showing a sharp main peak at approximately 6.911 minutes, indicating high purity with minimal secondary peaks.
Human Siglec-4a/MAG Protein (SIG-HM24A)
Human Siglec-4a/MAG Protein (SIG-HM24A)
SEC-HPLC chromatogram of recombinant human Siglec-4a/MAG protein showing a sharp main peak at approximately 6.911 minutes, indicating high purity with minimal secondary peaks.
Human Siglec-4a/MAG Protein (SIG-HM24A)
Human Siglec-4a/MAG Protein (SIG-HM24A)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gly20-Pro516
Catalog #SIG-HM24A
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Size
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Human Siglec-4a/MAG Protein (SIG-HM24A) Summary

Product Description

 Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly20-Pro516.

Product Background

 Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Siglec-4a/MAG Protein (SIG-HM24A) Specifications/Details

Protein
 Siglec-4a/MAG
Synonyms
 MAG; Siglec4a; Siglec-4a; S-MAG; GMA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly20-Pro516
Molecular Weight
 The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 500mM NaCl (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). Immobilized Human Siglec-4a, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
Human Siglec-4a on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Siglec-4a on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Human Siglec-4a is greater than 95% as determined by SEC-HPLC. The purity of-Human Siglec-4a is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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