• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
  • Amino Acid Range: Met17-Thr546
Catalog #SIG-HM411B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B) Summary

Product Description

 Recombinant Biotinylated Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Product Background

 Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411B) Specifications/Details

Protein
 Siglec-10
Synonyms
 SLG2; SIGLEC10; MGC126774; PRO940
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met17-Thr546
Molecular Weight
 The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tirs 150mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Biotinylated Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/Well) on the streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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