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Human Tenascin Protein (TNC-HM101) Summary
Product Description |
Recombinant Human Tenascin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly23-Ser621. |
Product Background |
Tenascin-C (TNC) is a hexameric, multimodular extracellular matrix protein with several molecular forms that are created through alternative splicing and protein modifications. It is highly conserved amongst vertebrates, and molecular phylogeny indicates that it evolved before fibronectin. Tenascin-C has many extracellular binding partners, including matrix components, soluble factors and pathogens; it also influences cell phenotype directly through interactions with cell surface receptors. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human Tenascin Protein (TNC-HM101) Specifications/Details
Protein |
Tenascin |
Synonyms |
TN; Hexabrachion; Tenascin-C; TN-C; TNC; Hxb |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly23-Ser621 |
Molecular Weight |
The protein has a predicted MW of 65.27 kDa. Due to glycosylation, the protein migrates to 66-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Tenascin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Tenascin is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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