Mouse TNFSF12/TWEAK Protein (TNF-MM612)

Express System: HEK293
Product Tag: N-Rabbit Fc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser46-His249

Catalog: TNF-MM612

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Product Description	Recombinant Mouse TNFSF12/TWEAK Protein is expressed from HEK293 with Rabbit Fc tag at the N-terminus.It contains Ser46-His249.
Product Background	Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
Product Category	Recombinant Protein

Target	TNFSF12/TWEAK
Gene/Protein ID	O54907
Species	Mouse
Exact Sequence	Ser46-His249
Molecular Weight	The protein has a predicted MW of 48.95 kDa. Due to furin cleavage and glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-Rabbit Fc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse TNFSF12, Rabbit Fc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
	Mouse TNFSF12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.