• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 64.7ng/ml determined by ELISA.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-CM101B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

 Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Biotinylated Cynomolgus TSLP Protein (Primary Amine Labeling) (TSP-CM101B) Specifications/Details

Protein
 TSLP
Synonyms
 thymic stromal lymphopoietin; TSLP
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Tyr29-Gln159
Molecular Weight
 The protein has a predicted MW of 16.3 kDa. Due to furin cleavage site, the protein migrates to 10-13 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 64.7ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 64.7ng/ml determined by ELISA. Immobilized Cynomolgus TSLPR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus TSLP, His Tag with the EC50 of 64.7ng/ml determined by ELISA.
Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%. Biotinylated Cynomolgus TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 13.40 nM as determined in BLI assay. Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 13.40 nM as determined in BLI assay.
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 1.12 nM as determined in BLI assay. Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus TSLP, His Tag with an affinity constant of 1.12 nM as determined in BLI assay.
The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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