Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-CM101B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117

Catalog: CDE-CM101B

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Product Description Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Asp117.

Product Background

 CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

 Recombinant Protein

Target

 CD3E/CD3 epsilon

Gene/Protein ID

 Q95LI5

Species

 Cynomolgus

Exact Sequence

 Gln22-Asp117
Molecular Weight The protein has a predicted MW of 11.7 kDa. Due to glycosylation, the protein migrates to 13-16 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E, His Tag with the EC50 of 0.87ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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