Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-CM110B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe19-Arg238

Catalog: PDL-CM110B

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Product Description Recombinant Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

 B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

 Recombinant Protein

Target

 PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

Gene/Protein ID

 G7PSE7

Species

 Cynomolgus/Rhesus macaque

Exact Sequence

 Phe19-Arg238
Molecular Weight The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus/Rhesus macaque PD-L1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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