Biotinylated Human BAFF/TNFSF13B/CD257 Protein (BAF-HM4AFB)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala134-Leu285

Catalog: BAF-HM4AFB

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Product Description Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala134-Leu285.

Product Background

 B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Product Category

 Recombinant Protein

Target

 BAFF/TNFSF13B/CD257

Gene/Protein ID

 Q9Y275-1

Species

 Human

Exact Sequence

 Ala134-Leu285
Molecular Weight The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-27 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human BAFFR, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA.
The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.
Biotinylated Human BAFF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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