Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-HM101B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp23-Asp126

Catalog: CDE-HM101B

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Product Description Recombinant Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Asp126.

Product Background

 CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

 Recombinant Protein

Target

 CD3E/CD3 epsilon

Gene/Protein ID

 P07766

Species

 Human

Exact Sequence

 Asp23-Asp126
Molecular Weight The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.9ng/ml determined by ELISA.
Biotinylated Human CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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