Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM467)
Catalog: MHC-HM467
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| Product Description | Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2), Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and RMFPNAPYL peptide. |
Product Background |
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer |
| MHC Form | Monomer |
| Allele | HLA-A*02:01 |
Gene/Protein ID |
A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&&P01887(Mouse B2M)&RMFPNAPYL |
Species |
Human&Mouse |
Exact Sequence |
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide |
| Molecular Weight | The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with dry ice. |
| Formulation | Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA. |
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The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC. |
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Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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