Cynomolgus CD200 R1/CRTR2 Protein (CD2-CM4R1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 35.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu25-Leu267

Catalog: CD2-CM4R1

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Product Description	Recombinant Cynomolgus CD200 R1/CRTR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Leu267.
Product Background	CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Product Category	Recombinant Protein

Target	CD200 R1/CRTR2
Gene/Protein ID	XP_005548207.1
Species	Cynomolgus
Exact Sequence	Glu25-Leu267
Molecular Weight	The protein has a predicted MW of 28.09 kDa. Due to glycosylation, the protein migrates to 67-77 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 35.84 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus CD200 R1, His Tag immobilized on CM5 Chip can bind Cynomolgus CD200, His Tag with an affinity constant of 35.84 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus CD200 R1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus CD200 R1 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.