Cynomolgus HGF R/c-MET Protein (MET-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu25-Asn930

Catalog: MET-CM101

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Product Description	Recombinant Cynomolgus HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Asn930.
Product Background	c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Product Category	Recombinant Protein

Target	HGF R/c-MET
Gene/Protein ID	A0A2K5UM36
Species	Cynomolgus
Exact Sequence	Glu25-Asn930
Molecular Weight	The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus HGF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA.
	The purity of Cynomolgus HGF R is greater than 95% as determined by SEC-HPLC.
	Cynomolgus HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.