Cynomolgus IL-10 R alpha Protein (IL1-CM1RA)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 35.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His22-Asn233

Catalog: IL1-CM1RA

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Product Description	Recombinant Cynomolgus IL-10 R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His22-Asn233.
Product Background	The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
Product Category	Recombinant Protein

Target	IL-10 R alpha
Gene/Protein ID	A0A2K5WJ66
Species	Cynomolgus
Exact Sequence	His22-Asn233
Molecular Weight	The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 35.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-10, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-10 R alpha, His Tag with the EC50 of 35.0ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.