Cynomolgus IL-3 Protein (IL3-CE101)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala20-Gln143

Catalog: IL3-CE101

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Cynomolgus IL-3 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala20-Gln143.
Product Background	Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Product Category	Recombinant Protein

Target	IL-3
Gene/Protein ID	G7P877
Species	Cynomolgus
Exact Sequence	Ala20-Gln143
Molecular Weight	The protein has a predicted MW of 15.13 kDa same as Tris-Bis PAGE result.
Activity	The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.