Human ANGPTL4/Angiopoietin-like 4 Protein (ANG-HM1L4)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 24.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Pro166-Ser406

Catalog: ANG-HM1L4

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Product Description Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro166-Ser406.

Product Background

Candidates for this common regulatory system include signals mediated by peroxisome proliferator-activated regulator and its response factor, angiopoietin-like 4. The expression and bioactivity of angiopoietin-like 4, an adipocytokine that was originally reported to have an angiogenic function, have been detected not only in the vascular system and adipose tissue but also in rheumatoid joints.

Product Category

Recombinant Protein

Target

ANGPTL4/Angiopoietin-like 4
Synonyms ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4; Angiopoietin-3; ANGPT4; MGC138181; MGC138183

Gene/Protein ID

Q9BY76-1

Species

Human

Exact Sequence

Pro166-Ser406
Molecular Weight The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 24.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human ANGPTL4, His Tag with an affinity constant of 24.50 nM as determined in SPR assay (Biacore T200).
The purity of Human Angiopoietin-like 4 is greater than 95% as determined by SEC-HPLC.
Human Angiopoietin-like 4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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