Human CD3E/CD3 epsilon Protein (CD3-HM20E)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human CD3E, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Asp23-Asp126(C119S, C122S)

Catalog: CD3-HM20E

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Product Description	Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S).
Product Background	T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein

Target	CD3E/CD3 epsilon
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Gene/Protein ID	P07766
Species	Human
Exact Sequence	Asp23-Asp126(C119S, C122S)
Molecular Weight	The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD3E, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD3E, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA (QC Test).
	Immobilized Human CD3E, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5/36.2/35.2ng/ml determined by ELISA.
	Human CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.