Mouse CD117 Protein (CD1-MM117)
Mouse CD117 Protein (CD1-MM117)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 14.02 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser25-Pro523
Catalog #CD1-MM117
Regular price $346.50
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Mouse CD117 Protein (CD1-MM117) Summary

Product Description

Recombinant Mouse CD117 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser25-Pro523.

Product Background

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse CD117 Protein (CD1-MM117) Specifications/Details

Protein
CD117
Synonyms
SCFR; PBT; CD117; ckit; c-kit; KIT
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ser25-Pro523
Molecular Weight
The protein has a predicted MW of 56.63 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 14.02 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 uM as determined in SPR assay (Biacore T200). Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 uM as determined in SPR assay (Biacore T200).
Mouse CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD117 is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD117 is greater than 95% as determined by SEC-HPLC.
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