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Rat Coagulation factor II-Protein (CFR-RM102) Summary
Product Description |
Recombinant Rat Coagulation factor II-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617. |
Product Background |
Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Rat Coagulation factor II-Protein (CFR-RM102) Specifications/Details
Protein |
Coagulation factor II |
Synonyms |
F2; Prothrombin; thrombin; F II; FII |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Gln25-Arg617 |
Molecular Weight |
The protein has a predicted MW of 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Rat Coagulation factor II on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Rat Coagulation factor II is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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